P-element 5' --> 3': TTTCATCATG motif   (at begining and end of p-elemnt)

 

Sau3AI Restriction Site: GATC  (Replaced BfuCI Restriction Site: GATC)

HpaII Restriction Site: CCGG 

 

+/+: Return sequence in database

+/- : Return reverse complement of what is in the database 

 

5' --> 3' sequenced with Sp1 (5' end of p-element)     

+/-: Coordinates decreasing, 5' --> 3' p-element orientation (add +1 to first nt match/duplicated nt: Reverse Complement)

 

+/+: Coordinates increasing,  3' -->5'   p-element orientation (add +8 to first nt match/duplicated nt: 1st 8nt)            

            

+/+: Coordinates increasing, 5' --> 3' p-element orientation (add +8 to first nt match/duplicated nt: 1st 8nt)    

+/-: Coordinates decreasing,  3' -->5'   p-element orientation (add +1 to first nt match/duplicated nt: Reverse Complement)

 

The are different orientations but both are Dove primers  so the Primer3 settings are:
Change NONE to Drosophila
Product size range: 601-700 701-850
Primer size: Min 18, Opt 23, Max 25
Tm Dove:  Min 51, Opt 53, Max 55


If you needed a Chris Primer the Primer3 settings are:
Change NONE to Drosophila
Product size range: 601-700 701-850
Primer size: Min 18, Opt 23, Max 25
Tm Chris:  Min 61, Opt 63, Max 65 

Mounting Adult Drosophila brains

 

Larval Brain Dissection in Drosophila melanogaster 

 

Larval Ring Gland Dissection: Isolation of Endocrine Organs from Drosophila